| Investigation of the coagulation system centers on the coagulation factors. The activity of these factors depends on specific surface receptors and phospholipids largely presented on the surface of the platelets and also by activated endothelium. The necessity for calcium in many of these reactions is frequently utilized to control their activity in Vitro. The APTT test is useful for measuring the activity of the intrinsic pathway. In APTT test, plasma is first preincubated for a specific period of time with a contact activator resulting in the formation of factor XIa. However coagulation cascade does not proceed further in the absence of calcium. Recalcification with Calcium chloride (0.025 mol/l) initiates the coagulation cascade resulting in the formation of a stable fibrin clot. The APTT test is influenced by the concentration of calcium chloride used for assessment of the intrinsic pathway. TM Calcium Chloride from Tulip is intended for use with APTT reagents. |
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