This procedure involves measurement of Creatine kinase (CK) activity in the presence of an antibody to CK-M monomer. CK-M fractions of the CKMM and the CK-MB in the sample are completely inhibited by an anti CK-M antibody present in the reagent. Then the activity of the CK-B fraction is measured by the CK (NAC act.) method. The CK-MB activity is obtained by multiplying the CK-B activity by two.
